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mouse refseq promoter arrays  (Arraystar inc)
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Arraystar inc mouse refseq promoter arrays
Mouse Refseq Promoter Arrays, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Expression of DNMTs and activity of Wnt signaling pathway were increased but Notum expression was decreased in laser-induced CNV mice. ( A – D ) Representative western blot and quantitative analysis of DNMT1, DNMT3A, and DNMT3B in REP/choroidal complexes from the indicated groups ( n = 9 mice/per group for all experiments). ( E – G ) qRT-PCR was performed to detect the mRNA expression of DNMTs in the RPE/choroidal complexes from normal and laser-induced CNV mice ( n = 6 mice/per group for all experiments). ( H ) Clustering of differentially enriched peaks <t>of</t> <t>methylation</t> in the <t>DNA</t> promoter region in the RPE/choroidal complexes from the normal group and the laser-induced CNV group ( red , hypermethylation; green , hypomethylation). ( I ) Enrichment analysis of KEGG pathways and the top 10 activated pathways identified among hypermethylated differentially methylated genes in the RPE/choroidal complexes from the normal group and the laser-induced CNV group. ( J – P ) qRT-PCR was performed to detect the mRNA expression of Wnt signaling-related genes in the RPE/choroidal complexes from the normal group and the laser-induced CNV group ( n = 9 mice/per group for all experiments). Each experiment was repeated at least three times, and data in the graphs represent the mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001.
Mouse Dna Promoter Array, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse refseq promoter methylation array 4 × 180 k  (Arraystar inc)
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Expression of DNMTs and activity of Wnt signaling pathway were increased but Notum expression was decreased in laser-induced CNV mice. ( A – D ) Representative western blot and quantitative analysis of DNMT1, DNMT3A, and DNMT3B in REP/choroidal complexes from the indicated groups ( n = 9 mice/per group for all experiments). ( E – G ) qRT-PCR was performed to detect the mRNA expression of DNMTs in the RPE/choroidal complexes from normal and laser-induced CNV mice ( n = 6 mice/per group for all experiments). ( H ) Clustering of differentially enriched peaks <t>of</t> <t>methylation</t> in the <t>DNA</t> promoter region in the RPE/choroidal complexes from the normal group and the laser-induced CNV group ( red , hypermethylation; green , hypomethylation). ( I ) Enrichment analysis of KEGG pathways and the top 10 activated pathways identified among hypermethylated differentially methylated genes in the RPE/choroidal complexes from the normal group and the laser-induced CNV group. ( J – P ) qRT-PCR was performed to detect the mRNA expression of Wnt signaling-related genes in the RPE/choroidal complexes from the normal group and the laser-induced CNV group ( n = 9 mice/per group for all experiments). Each experiment was repeated at least three times, and data in the graphs represent the mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001.
Mouse Refseq Promoter Methylation Array 4 × 180 K, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse refseq promoter methylation array 4 × 180k  (Arraystar inc)
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Expression of DNMTs and activity of Wnt signaling pathway were increased but Notum expression was decreased in laser-induced CNV mice. ( A – D ) Representative western blot and quantitative analysis of DNMT1, DNMT3A, and DNMT3B in REP/choroidal complexes from the indicated groups ( n = 9 mice/per group for all experiments). ( E – G ) qRT-PCR was performed to detect the mRNA expression of DNMTs in the RPE/choroidal complexes from normal and laser-induced CNV mice ( n = 6 mice/per group for all experiments). ( H ) Clustering of differentially enriched peaks <t>of</t> <t>methylation</t> in the <t>DNA</t> promoter region in the RPE/choroidal complexes from the normal group and the laser-induced CNV group ( red , hypermethylation; green , hypomethylation). ( I ) Enrichment analysis of KEGG pathways and the top 10 activated pathways identified among hypermethylated differentially methylated genes in the RPE/choroidal complexes from the normal group and the laser-induced CNV group. ( J – P ) qRT-PCR was performed to detect the mRNA expression of Wnt signaling-related genes in the RPE/choroidal complexes from the normal group and the laser-induced CNV group ( n = 9 mice/per group for all experiments). Each experiment was repeated at least three times, and data in the graphs represent the mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001.
Mouse Refseq Promoter Methylation Array 4 × 180k, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse promoter arrays  (Agilent technologies)
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Expression of DNMTs and activity of Wnt signaling pathway were increased but Notum expression was decreased in laser-induced CNV mice. ( A – D ) Representative western blot and quantitative analysis of DNMT1, DNMT3A, and DNMT3B in REP/choroidal complexes from the indicated groups ( n = 9 mice/per group for all experiments). ( E – G ) qRT-PCR was performed to detect the mRNA expression of DNMTs in the RPE/choroidal complexes from normal and laser-induced CNV mice ( n = 6 mice/per group for all experiments). ( H ) Clustering of differentially enriched peaks <t>of</t> <t>methylation</t> in the <t>DNA</t> promoter region in the RPE/choroidal complexes from the normal group and the laser-induced CNV group ( red , hypermethylation; green , hypomethylation). ( I ) Enrichment analysis of KEGG pathways and the top 10 activated pathways identified among hypermethylated differentially methylated genes in the RPE/choroidal complexes from the normal group and the laser-induced CNV group. ( J – P ) qRT-PCR was performed to detect the mRNA expression of Wnt signaling-related genes in the RPE/choroidal complexes from the normal group and the laser-induced CNV group ( n = 9 mice/per group for all experiments). Each experiment was repeated at least three times, and data in the graphs represent the mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001.
Mouse Promoter Arrays, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Expression of DNMTs and activity of Wnt signaling pathway were increased but Notum expression was decreased in laser-induced CNV mice. ( A – D ) Representative western blot and quantitative analysis of DNMT1, DNMT3A, and DNMT3B in REP/choroidal complexes from the indicated groups ( n = 9 mice/per group for all experiments). ( E – G ) qRT-PCR was performed to detect the mRNA expression of DNMTs in the RPE/choroidal complexes from normal and laser-induced CNV mice ( n = 6 mice/per group for all experiments). ( H ) Clustering of differentially enriched peaks <t>of</t> <t>methylation</t> in the <t>DNA</t> promoter region in the RPE/choroidal complexes from the normal group and the laser-induced CNV group ( red , hypermethylation; green , hypomethylation). ( I ) Enrichment analysis of KEGG pathways and the top 10 activated pathways identified among hypermethylated differentially methylated genes in the RPE/choroidal complexes from the normal group and the laser-induced CNV group. ( J – P ) qRT-PCR was performed to detect the mRNA expression of Wnt signaling-related genes in the RPE/choroidal complexes from the normal group and the laser-induced CNV group ( n = 9 mice/per group for all experiments). Each experiment was repeated at least three times, and data in the graphs represent the mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001.
Genechip Mouse Promoter 1.0r Arrays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Expression of DNMTs and activity of Wnt signaling pathway were increased but Notum expression was decreased in laser-induced CNV mice. ( A – D ) Representative western blot and quantitative analysis of DNMT1, DNMT3A, and DNMT3B in REP/choroidal complexes from the indicated groups ( n = 9 mice/per group for all experiments). ( E – G ) qRT-PCR was performed to detect the mRNA expression of DNMTs in the RPE/choroidal complexes from normal and laser-induced CNV mice ( n = 6 mice/per group for all experiments). ( H ) Clustering of differentially enriched peaks <t>of</t> <t>methylation</t> in the <t>DNA</t> promoter region in the RPE/choroidal complexes from the normal group and the laser-induced CNV group ( red , hypermethylation; green , hypomethylation). ( I ) Enrichment analysis of KEGG pathways and the top 10 activated pathways identified among hypermethylated differentially methylated genes in the RPE/choroidal complexes from the normal group and the laser-induced CNV group. ( J – P ) qRT-PCR was performed to detect the mRNA expression of Wnt signaling-related genes in the RPE/choroidal complexes from the normal group and the laser-induced CNV group ( n = 9 mice/per group for all experiments). Each experiment was repeated at least three times, and data in the graphs represent the mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001.
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Expression of DNMTs and activity of Wnt signaling pathway were increased but Notum expression was decreased in laser-induced CNV mice. ( A – D ) Representative western blot and quantitative analysis of DNMT1, DNMT3A, and DNMT3B in REP/choroidal complexes from the indicated groups ( n = 9 mice/per group for all experiments). ( E – G ) qRT-PCR was performed to detect the mRNA expression of DNMTs in the RPE/choroidal complexes from normal and laser-induced CNV mice ( n = 6 mice/per group for all experiments). ( H ) Clustering of differentially enriched peaks <t>of</t> <t>methylation</t> in the <t>DNA</t> promoter region in the RPE/choroidal complexes from the normal group and the laser-induced CNV group ( red , hypermethylation; green , hypomethylation). ( I ) Enrichment analysis of KEGG pathways and the top 10 activated pathways identified among hypermethylated differentially methylated genes in the RPE/choroidal complexes from the normal group and the laser-induced CNV group. ( J – P ) qRT-PCR was performed to detect the mRNA expression of Wnt signaling-related genes in the RPE/choroidal complexes from the normal group and the laser-induced CNV group ( n = 9 mice/per group for all experiments). Each experiment was repeated at least three times, and data in the graphs represent the mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001.
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Expression of DNMTs and activity of Wnt signaling pathway were increased but Notum expression was decreased in laser-induced CNV mice. ( A – D ) Representative western blot and quantitative analysis of DNMT1, DNMT3A, and DNMT3B in REP/choroidal complexes from the indicated groups ( n = 9 mice/per group for all experiments). ( E – G ) qRT-PCR was performed to detect the mRNA expression of DNMTs in the RPE/choroidal complexes from normal and laser-induced CNV mice ( n = 6 mice/per group for all experiments). ( H ) Clustering of differentially enriched peaks of methylation in the DNA promoter region in the RPE/choroidal complexes from the normal group and the laser-induced CNV group ( red , hypermethylation; green , hypomethylation). ( I ) Enrichment analysis of KEGG pathways and the top 10 activated pathways identified among hypermethylated differentially methylated genes in the RPE/choroidal complexes from the normal group and the laser-induced CNV group. ( J – P ) qRT-PCR was performed to detect the mRNA expression of Wnt signaling-related genes in the RPE/choroidal complexes from the normal group and the laser-induced CNV group ( n = 9 mice/per group for all experiments). Each experiment was repeated at least three times, and data in the graphs represent the mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001.

Journal: Investigative Ophthalmology & Visual Science

Article Title: 5-Aza-2′-Deoxycytidine Ameliorates Choroidal Neovascularization by Inhibiting the Wnt/β-Catenin Signaling Pathway

doi: 10.1167/iovs.65.2.23

Figure Lengend Snippet: Expression of DNMTs and activity of Wnt signaling pathway were increased but Notum expression was decreased in laser-induced CNV mice. ( A – D ) Representative western blot and quantitative analysis of DNMT1, DNMT3A, and DNMT3B in REP/choroidal complexes from the indicated groups ( n = 9 mice/per group for all experiments). ( E – G ) qRT-PCR was performed to detect the mRNA expression of DNMTs in the RPE/choroidal complexes from normal and laser-induced CNV mice ( n = 6 mice/per group for all experiments). ( H ) Clustering of differentially enriched peaks of methylation in the DNA promoter region in the RPE/choroidal complexes from the normal group and the laser-induced CNV group ( red , hypermethylation; green , hypomethylation). ( I ) Enrichment analysis of KEGG pathways and the top 10 activated pathways identified among hypermethylated differentially methylated genes in the RPE/choroidal complexes from the normal group and the laser-induced CNV group. ( J – P ) qRT-PCR was performed to detect the mRNA expression of Wnt signaling-related genes in the RPE/choroidal complexes from the normal group and the laser-induced CNV group ( n = 9 mice/per group for all experiments). Each experiment was repeated at least three times, and data in the graphs represent the mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: In this study, the Arraystar Mouse DNA Promoter Array was utilized to determine methylation profiles in the whole genome in the DNA promoters of the RPE/choroidal complex in mice 7 days after laser photocoagulation because the optimum time point to assess CNV formation is typically at day 7 to day 14.

Techniques: Expressing, Activity Assay, Western Blot, Quantitative RT-PCR, Methylation